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  • Single-Cell RNA Detection to Identify Cell Subpopulations in Heterogeneous Mixtures

    Single-Cell RNA Detection to Identify Cell Subpopulations in Heterogeneous Mixtures

    Tuesday, March 25, 2014
    Application Note
    Don Weldon, Victor Koong, Alex Ko, Yuko Williams, Laura White
    Traditional RNA detection methods, which analyze RNA levels in lysed, permeabilized, or fixed cell samples, make it challenging to understand the role of one gene in the context of a tissue, organ, or... read more
  • Micro RNA Profiling with a Multiplexed Assay

    Micro RNA Profiling with a Multiplexed Assay

    Monday, March 03, 2014
    Application Note
    Mark Santos
    From the discovery of new miRNAs to clarifying their biological roles and utility as biomarkers, micro RNA (miRNA)-based research is undergoing a period of change. Rigorous methods are therefore ... read more
  • Identification of Cancer Stem Cells Within Mixed Populations Using Live Cell RNA Detection

    Identification of Cancer Stem Cells Within Mixed Populations Using Live Cell RNA Detection

    Thursday, January 09, 2014
    Application Note
    Don Weldon, Steve McClellan
    Despite the ubiquity of studies measuring mRNA and miRNA levels, many current techniques require cell lysis and are enormously disruptive to the cells being studied. read more
  • Improved Long-Term Culture Using A Microfluidic Platform

    Improved Long-Term Culture Using A Microfluidic Platform

    Monday, October 07, 2013
    Application Note
    Alex Mok, Paul Hung, Cindy Chen, Sónia Gil, Kathleen Ongena
    Precise and biologically relevant in vitro studies require the ability to analyze cell responses to perturbations over time. In order to achieve successful long-term cell culture and imaging, however,... read more
  • Multiparameter Pluripotency Analysis Using Magnetic Bead Assays

    Multiparameter Pluripotency Analysis Using Magnetic Bead Assays

    Wednesday, August 28, 2013
    Application Note
    Nikolai Stankiewicz, Thomas Siegl, Frederick Wiese
    For the application of induced pluripotent stem cells (iPSCs) and human embryonic stem cells (hESCs) to regenerative medicine and drug discovery research, it is crucial to characterize the identity ... read more
  • Measurement of IL-6 RNA Levels in Live Cells

    Measurement of IL-6 RNA Levels in Live Cells

    Wednesday, July 10, 2013
    Application Note
    Shobana Vaidyanathan
    Infections and tissue damage lead to acute inflammatory responses, orchestrated by the release of cytokines from macrophages and mast cells, as well as endothelium and tumor cells. Tumor necrosis ... read more
  • Fast, Efficient Antibody Biotinylation for Cleaner Immunodetection Assays

    Fast, Efficient Antibody Biotinylation for Cleaner Immunodetection Assays

    Friday, June 07, 2013
    Application Note
    Amedeo Cappione, Masaharu Mabuchi, David Briggs, Timothy Nadler
    Many protein detection methods, including Western blotting, flow cytometry, ELISA, and immunohistochemistry, require the use of target-specific probes, such as antibodies, that are detected via two-... read more
  • Detection of siRNA-Mediated Gene Modulation Using RNA Detection Probes

    Detection of siRNA-Mediated Gene Modulation Using RNA Detection Probes

    Monday, May 20, 2013
    Application Note
    Don Weldon, Grace Johnston
    To study the function of genes and biological pathways, researchers often use techniques such as RNA interference (RNAi) to modify gene expression. In RNAi experiments, it is important to determine ... read more
  • Pittcon® 2012: Laboratory Instrumentation and Equipment Product Showcase

    Pittcon® 2012: Laboratory Instrumentation and Equipment Product Showcase

    Monday, May 07, 2012
    Technical Article
    Jeanely Hunt, MS, MBA
    Pittcon® 2012 was held March 11–15, 2012 at the Orange County Convention Center in Orlando, Florida. The following product showcase provides a brief outline of new and current laboratory equipment and... read more
  • Use of Automated Solid-Phase Extraction and GC-MS/MS to Evaluate Nitrosamines in Water Matrices

    Use of Automated Solid-Phase Extraction and GC-MS/MS to Evaluate Nitrosamines in Water Matrices

    Tuesday, March 06, 2012
    Technical Article
    Janie C. Holady, Rebecca A. Trenholm, Shane A. Snyder
    An analytical method for detecting nitrosamines was demonstrated in various water matrices: finished drinking, surface, and treated wastewater effluent. read more
  • Optimizing HPLC Sample Preparation

    Optimizing HPLC Sample Preparation

    Monday, February 06, 2012
    Technical Article
    Caitlin Smith
    Sample preparation is an important component for analysis in high-performance liquid chromatography (HPLC)/ultra high-performance liquid chromatography (UHPLC). This article outlines commonly used ... read more
  • Matrix Degradation Assays for Investigating Cell Invasion

    Matrix Degradation Assays for Investigating Cell Invasion

    Tuesday, November 01, 2011
    Application Note
    Jun Ma, Luke Armstrong, Janet Anderl
    Tumor metastasis, inflammation, and development are accompanied by the invasion of cells through the extracellular matrix. read more
  • Focus on Columns and Consumables at Pittcon® 2011

    Focus on Columns and Consumables at Pittcon® 2011

    Tuesday, May 10, 2011
    Technical Article
    Robert L. Stevenson
    Columns, chemicals, and consumables (CCCs) account for more than 30% of the chromatography market. read more
  • Cellular Reprogramming: Opportunities and Challenges

    Cellular Reprogramming: Opportunities and Challenges

    Monday, May 09, 2011
    Application Note
    Vi Chu, Amy Noble, Louise Rollins
    In 2007, researchers created the first human induced pluripotent stem cells (iPS cells) by infecting skin cells with four transcription factors (Oct-4, Klf4, Sox-2, and c-Myc). read more
  • General Laboratory Equipment: Innovation Showcase

    General Laboratory Equipment: Innovation Showcase

    Monday, May 09, 2011
    Technical Article
    Jeanely Hunt, MS, MBA
    Each year, manufacturers and distributors flesh out many innovations for the laboratory, ranging from simple accessories to large, more complex instrumentation. read more
  • Pittcon® 2011 Editors’ Awards: A Two-Way Tie for the Gold Award

    Pittcon® 2011 Editors’ Awards: A Two-Way Tie for the Gold Award

    Friday, April 01, 2011
    Technical Article
    Jeanely Hunt, MS, MBA
    Pittcon 2011 was held March 13–18, 2011 at the Georgia World Congress Center in Atlanta, GA. The exhibition displayed an assortment of laboratory products, including anything from small plastic ... read more
  • New Products at Pittcon® 2011

    New Products at Pittcon® 2011

    Thursday, March 10, 2011
    Application Note
    Jeanely Hunt, MS, MBA
    The following includes some of the latest advances in laboratory instrumentation debuting at Pittcon® 2011. read more
  • Analysis of Piperine in Peppers Using On-Line SFE-UHPLC With Photodiode Array Detection

    Analysis of Piperine in Peppers Using On-Line SFE-UHPLC With Photodiode Array Detection

    Tuesday, February 01, 2011
    Application Note
    Takayuki Yamaguchi, Kazutoshi Kamezawa, Keijin Iwaya, Yasuyo Sato, Toshihiko Miyaji, Masao Bounoshita, David J. Tognarelli, Muneo Saito
    Ultrahigh-performance liquid chromatography (UHPLC) is a separation technique that utilizes columns packed with microparticles (<2 µm in diameter), as provided by the xtreme liquid chromatography (x-lc™) system (jasco corp., tokyo, japan) µm="" in="" diameter),="" as="" provided="" by="" the="" xtreme="" liquid="" chromatography="" (x-lc™)="" system="" (jasco="" corp.,="" tokyo,="">... read more
  • The Double Life of Structured Fluids

    The Double Life of Structured Fluids

    Sunday, August 01, 2010
    Application Note
    Neil Cunningham, Bob McGregor
    The products we stir, pump, spread, pour, and spray are usually thought of as liquids. Surprisingly, however, it can be demonstrated that they live a quiet double life as liquids when observed,but ... read more
  • Food Texture Analysis

    Food Texture Analysis

    Saturday, May 01, 2010
    Application Note
    Richard D. Brown
    Texture testing is a well-established technique for evaluating the mechanical and physical properties of raw ingredients, food structure, and designs, and for pre- and post-quality control checks. ... read more
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