Optimizing HPLC Sample Preparation

Sample preparation is an important component for analysis in high-performance liquid chromatography (HPLC)/ultra high-performance liquid chromatography (UHPLC). This article outlines commonly used sample preparation techniques—centrifugation, liquid–liquid extraction, and solid-phase extraction (SPE). 

The most effective of these techniques is SPE. Disposable SPE cartridges, automated SPE systems, and bonded silica devices for HPLC and UHPLC preparation make solid-phase extraction an optimal method for sample preparation.

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