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  • An Improved Method to Generate Virus-Free Human iPSCs

    An Improved Method to Generate Virus-Free Human iPSCs

    Monday, November 03, 2014
    Application Note
    Vi Chu, Nick Asbrock, Min Lu
    Induced pluripotent stem cells (iPSCs) are offering new opportunities in both research and clinical applications. Researchers are using these cells for drug and toxicity screening, as well as for ... read more
  • Meeting the Challenge of Biological Changes Postsampling

    Meeting the Challenge of Biological Changes Postsampling

    Friday, May 16, 2014
    Application Note
    Marcus Söderquist
    Understanding the role of proteins, peptides, and their post-translational modifications, e.g., phosphorylations, in normal and diseased biological samples is crucial to elucidate in vivo biological ... read more
  • Quality Control: An Important Success Factor in Nucleic Acid-Based Analysis

    Quality Control: An Important Success Factor in Nucleic Acid-Based Analysis

    Tuesday, April 01, 2014
    Application Note
    Carola Schade
    Quality control (QC) is becoming increasingly important in nucleic acid-based testing. As detection technologies become more and more sensitive, final results are influenced not only by biological ... read more
  • Single-Cell RNA Detection to Identify Cell Subpopulations in Heterogeneous Mixtures

    Single-Cell RNA Detection to Identify Cell Subpopulations in Heterogeneous Mixtures

    Tuesday, March 25, 2014
    Application Note
    Don Weldon, Victor Koong, Alex Ko, Yuko Williams, Laura White
    Traditional RNA detection methods, which analyze RNA levels in lysed, permeabilized, or fixed cell samples, make it challenging to understand the role of one gene in the context of a tissue, organ, or... read more
  • Micro RNA Profiling with a Multiplexed Assay

    Micro RNA Profiling with a Multiplexed Assay

    Monday, March 03, 2014
    Application Note
    Mark Santos
    From the discovery of new miRNAs to clarifying their biological roles and utility as biomarkers, micro RNA (miRNA)-based research is undergoing a period of change. Rigorous methods are therefore ... read more
  • Improved Long-Term Culture Using A Microfluidic Platform

    Improved Long-Term Culture Using A Microfluidic Platform

    Monday, October 07, 2013
    Application Note
    Alex Mok, Paul Hung, Cindy Chen, Sónia Gil, Kathleen Ongena
    Precise and biologically relevant in vitro studies require the ability to analyze cell responses to perturbations over time. In order to achieve successful long-term cell culture and imaging, however,... read more
  • Impact of New Pipetting Techniques on Safety in the Laboratory

    Impact of New Pipetting Techniques on Safety in the Laboratory

    Monday, September 02, 2013
    Application Note
    Bobby Chavli, Annette Summers, Mary Napier
    According to the Occupational Safety and Health Administration (OSHA), on average, it takes between 18 and 36 months to develop and implement a new regulation, trailing the pace of science. As a ... read more
  • DNA in Forensics

    DNA in Forensics

    Wednesday, August 28, 2013
    Technical Article
    Robert L. Stevenson, Ph.D.
    The proliferation of forensic DNA testing and databases by state and local jurisdictions was recently reported by Joseph Goldstein in The New York Times. For years, the FBI’s combined indexing system ... read more
  • Multiparameter Pluripotency Analysis Using Magnetic Bead Assays

    Multiparameter Pluripotency Analysis Using Magnetic Bead Assays

    Wednesday, August 28, 2013
    Application Note
    Nikolai Stankiewicz, Thomas Siegl, Frederick Wiese
    For the application of induced pluripotent stem cells (iPSCs) and human embryonic stem cells (hESCs) to regenerative medicine and drug discovery research, it is crucial to characterize the identity ... read more
  • High-Resolution Separation of Nucleic Acid Fragments

    High-Resolution Separation of Nucleic Acid Fragments

    Wednesday, August 07, 2013
    Application Note
    Carola Schade
    The invention of the polymerase chain reaction (PCR) in the early 1980s revolutionized molecular biology and molecular medicine. Since then, PCR has developed into a widely used technique for ... read more
  • A Capillary Gel Electrophoresis Fluorescence System for Antibody–Antigen Interactions

    A Capillary Gel Electrophoresis Fluorescence System for Antibody–Antigen Interactions

    Wednesday, August 07, 2013
    Application Note
    Shou-Kuan Tsai, Ming-Jhy Hseu, Varoujan D. Amirkhanian
    Since its inception more than 20 years ago, slab gel-based electrophoresis technology has been used routinely for the analysis of biomolecules (i.e., DNA, proteins, and carbohydrates). However, use of... read more
  • Translational Medicine and the ’Omics Electronic Lab Notebooks

    Translational Medicine and the ’Omics Electronic Lab Notebooks

    Tuesday, August 06, 2013
    Technical Article
    Chris Molloy
    Translational medicine research (TransMed) is a new environment in which groups of stakeholders come together to review increasingly complex data with the goals of improving outcomes, getting more ... read more
  • The Role of Synthetic DNA in Big DNA and Genetics Research

    The Role of Synthetic DNA in Big DNA and Genetics Research

    Friday, August 02, 2013
    Technical Article
    Dan Gibson, Hans Packer
    Synthetic biology is defined as the de novo design and construction of biological devices and systems, and as such is heavily reliant on the use of synthetic DNA. The size and sequence of potential ... read more
  • Measurement of IL-6 RNA Levels in Live Cells

    Measurement of IL-6 RNA Levels in Live Cells

    Wednesday, July 10, 2013
    Application Note
    Shobana Vaidyanathan
    Infections and tissue damage lead to acute inflammatory responses, orchestrated by the release of cytokines from macrophages and mast cells, as well as endothelium and tumor cells. Tumor necrosis ... read more
  • High-Throughput Screening of Multiple Protein Complexes

    High-Throughput Screening of Multiple Protein Complexes

    Thursday, July 04, 2013
    Application Note
    Imre Berger, Maxime Chaillet, Frederic Garzoni, Sinyee Yau-Rose, Barney Zoro
    Key protein complexes are fundamental to a wide range of cellular processes and key metabolic pathways within the body. Their dysfunction is associated with a range of disease pathologies such as ... read more
  • Product Intelligence: Thermal Cyclers: Behind the Technology

    Product Intelligence: Thermal Cyclers: Behind the Technology

    Friday, June 28, 2013
    Technical Article
    Caitlin Smith
    Also referred to as thermocyclers, DNA amplifiers, or PCR machines, thermal cyclers are a vital part of the molecular biology laboratory. Many choices of thermal cycler are available, and this article... read more
  • Fast, Efficient Antibody Biotinylation for Cleaner Immunodetection Assays

    Fast, Efficient Antibody Biotinylation for Cleaner Immunodetection Assays

    Friday, June 07, 2013
    Application Note
    Amedeo Cappione, Masaharu Mabuchi, David Briggs, Timothy Nadler
    Many protein detection methods, including Western blotting, flow cytometry, ELISA, and immunohistochemistry, require the use of target-specific probes, such as antibodies, that are detected via two-... read more
  • Detection of siRNA-Mediated Gene Modulation Using RNA Detection Probes

    Detection of siRNA-Mediated Gene Modulation Using RNA Detection Probes

    Monday, May 20, 2013
    Application Note
    Don Weldon, Grace Johnston
    To study the function of genes and biological pathways, researchers often use techniques such as RNA interference (RNAi) to modify gene expression. In RNAi experiments, it is important to determine ... read more
  • Putting the “More” in Morphology: Part 2

    Putting the “More” in Morphology: Part 2

    Wednesday, April 10, 2013
    Technical Article
    Barbara Foster
    Part 1 of this article discussed the impact of multiplexing morphological methods, multiparametric techniques, and supervised machine-learning to answer the question "what" is there, as well as "where... read more
  • Optimizing the Western Blotting Procedure: Solutions for Faster Western Blots

    Optimizing the Western Blotting Procedure: Solutions for Faster Western Blots

    Monday, July 30, 2012
    Application Note
    Catherine Shaffer
    Advances in technology make the Western blotting procedure both faster and quantitative. Solutions for faster Western blots include faster transfer equipment and faster reagents. Purchasing a ... read more
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