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A Microfluidics-Based Droplet Plate Reader for Nucleic Acid and Protein Quantification
Life science laboratories are gradually moving toward automated liquid handling to achieve cost reduction, productivity increases, and improved data consistency and quality.
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The Interaction Between Gemini Surfactant and Bovine Serum Albumin
In recent years, Gemini surfactants have emerged as promising surface active agents.
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HPLC Application Note: U.S. EPA Priority Pollutants in Water
Most basic recommended instrument: Cecil Adept System 4S.
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HPLC Application Note: Ultralow Detection Limits for Inorganic Anions and Cations in Power Station Boiler Water Analysis
Boilers used in power stations hold ultrapure water, and the purity of that water must be determined by highly sensitive techniques.
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A Generic Method for the Analysis of Residual Solvents in Pharmaceuticals Using Static Headspace-GC-FID-MS
The determination of residual solvents (RS), formerly called organic volatile impurities (OVI), in pharmaceutical products is probably the most important application of gas chromatography in ...
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An Advanced Base-Deactivated Capillary Column for Analysis of Volatile Amines, Ammonia, and Alcohols
To analyze basic compounds at nanogram levels using gas chromatography, a basic surface modification is often required to reduce the impact of the acidic fused silica.
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Staistics in Analytical Chemistry: Part 28—Statistically Derived Detection Limits
In the previous article (Part 27, American Laboratory, Sept 2007), the world of detection was shown to revolve around three variables: 1) the detection limit, 2) the probability of false positives, a,...
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Statistics in Analytical Chemistry: Part 29—Statistically Derived Detection Limits (continued)
Part 28 (American Laboratory, Nov/Dec 2007) laid the framework for discussing detection limits. The relationship among the detection limit (DL), the probability of false positives (a), and the ...
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Statistics in Analytical Chemistry: Part 30—Statistically Derived Detection Limits (Concluded)
In Part 29 (American Laboratory, Feb 2008), Hubaux-Vos detection limits (H-V DLs) were explained and an illustration was given.
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Determination of Enantiomeric Purity of a-Bromobutyric Acid by HPLC Combined With Pre-Column Derivatization
As a common intermediate in the synthesis of numerous pharmaceuticals, a-bromobutyric acid displays chirality at the carbon bound to the bromine.
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Statistics in Analytical Chemistry: Part 31—Receiver Operating Characteristic (ROC) Curves: Equations
Please do not despair! This article is not a series of formulas to be memorized.
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Extending the Scope of Microwave-Assisted Method Development
In the R&D laboratories of universities and the pharmaceutical industry, microwave synthesis is becoming increasingly popular.
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Use of High-Resolution MS for Discovery Pharmocokinetics and Simultaneous Analysis of Metabolites
It is essential to eliminate poor drug candidates as early as possible in the drug development process. Unfavorable pharmacokinetic (PK) properties have frequently been the reason for failure of new ...
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Statistics in Analytical Chemistry: Part 32—Detection Limits Via 3-Sigma
As was mentioned in the first article on detection limits (DLs) (Part 26, American Laboratory, June/July 2007), many formulas exist for calculating these values.
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Statistics in Analytical Chemistry: Part 33—Detection Limits via 3-Sigma (Concluded)
In the last installment (Part 32, American Laboratory, Nov/Dec 2008) of this series, three detection limits (DLs) were calculated (via the 3-Sigma [3s] approach, where a is set to be 0.01) for a ...
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