Speeding up Glycan Analysis without Compromising Resolution Using High-resolution Ion Mobility

 Speeding up Glycan Analysis without Compromising Resolution Using High-resolution Ion Mobility

by Steven Broome (MOBILion Systems), Philip Yoo (GlaxoSmithKline), Dmitry Avtonomov (Protein Metrics Inc), Andrew Nichols (Protein Metrics Inc), Abhishek Roushan (Protein Metrics Inc) and Rose Lawler (Protein Metrics Inc)

Many protein-based therapeutics contain glycans as part of their structure, requiring thorough characterization and systematic batch control. However, as mass spectrometry instrumentation has become faster and more sensitive, the time and resolution of such analyses have become severely limited by the liquid chromatography (LC) methods used for separation. In this study, we demonstrate how these difficulties can be overcome by coupling hydrophilic interaction liquid chromatography (HILIC) with high-resolution ion mobility-mass spectrometry (HRIM-MS) and streamlined software, to comprehensively analyze N-glycans with substantially shorter HILIC run times, without compromising resolution.

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