Preparative high performance liquid chromatography (HPLC), like its analytical counterpart, provides fast and sensitive separations of mixtures using high pressures and flow rates. Preparative HPLC is a valuable technique for purifying components from even complex mixtures, such as drug ingredients, food additives and other valuable industrial compounds. While utilizing the same selective separation methodology and much of the same instrumentation as analytical HPLC, preparative HPLC allows purified target compounds to be collected separately after elution and also requires different column dimensions and run parameters to achieve the desired result. The following tips can help improve your purification HPLC workflows, from strategies in method development and scale up to general best practices.
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