This article outlines the components of an HPLC system, from solvent reservoir to solvent pump to injector, column and data processing unit. Types and applications of HPLC instruments are provided, that is, basic HPLC systems; research-grade units; automated, clinical-grade systems; and industrial-grade, workhorse instruments. The text also lists commonly used HPLC techniques and their principles of operation, as well as important purchasing considerations.
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