Next gen sequencing (NGS) has substantially evolved since its earliest developments back in the early 1970s. Frederick Sanger discovered and coined the first sequencing method as the Sanger Method, which is also commonly referred to as the chain-terminator or dideoxy sequencing method that utilizes a cocktail of 2',3'-dideoxynucleotide triphospates (ddNTPs). The need for affordable sequencing led to various high-throughput sequencing methods, such as massively parallel signature sequencing (MPSS), polony sequencing, 454 pyrosequencing, single molecule real time (RNAP) sequencing, and many more.
Latest NGS trends in the market
- Price of NGS sequencing and synthesis instruments and what it costs to run them is dropping
- Moving into hospitals and diagnostic labs to test for rare, difficult-to-diagnose disorders, such as mitochondrial diseases; and cancers, such as the BRCA1/2 mutations and the multiple variants
Let's hear from you:
- How often do you perform Next Gen Sequencing (NGS)?
- What are the applications of your Next Gen Sequencing (NGS) experiments?
- Do you automate any step of the NGS sample prep process?
Take this 5 minute survey on Next Gen Sequencing:
Eligible participants must use a Next Gen Sequencer of any brand.