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  • Putting the “More” in Morphology: Part 2

    Wednesday, April 10, 2013
    Technical Article
    Barbara Foster
    Part 1 of this article discussed the impact of multiplexing morphological methods, multiparametric techniques, and supervised machine-learning to answer the question "what" is there, as well as "where... read more

  • Detection of siRNA-Mediated Gene Modulation Using RNA Detection Probes

    Monday, May 20, 2013
    Application Note
    Don Weldon Grace Johnston
    To study the function of genes and biological pathways, researchers often use techniques such as RNA interference (RNAi) to modify gene expression. In RNAi experiments, it is important to determine ... read more

  • Fast, Efficient Antibody Biotinylation for Cleaner Immunodetection Assays

    Friday, June 07, 2013
    Application Note
    Amedeo Cappione Masaharu Mabuchi David Briggs Timothy Nadler
    Many protein detection methods, including Western blotting, flow cytometry, ELISA, and immunohistochemistry, require the use of target-specific probes, such as antibodies, that are detected via two-... read more

  • High-Throughput Screening of Multiple Protein Complexes

    Thursday, July 04, 2013
    Application Note
    Imre Berger Maxime Chaillet Frederic Garzoni Sinyee Yau-Rose Barney Zoro
    Key protein complexes are fundamental to a wide range of cellular processes and key metabolic pathways within the body. Their dysfunction is associated with a range of disease pathologies such as ... read more

  • Measurement of IL-6 RNA Levels in Live Cells

    Wednesday, July 10, 2013
    Application Note
    Shobana Vaidyanathan
    Infections and tissue damage lead to acute inflammatory responses, orchestrated by the release of cytokines from macrophages and mast cells, as well as endothelium and tumor cells. Tumor necrosis ... read more

  • Multiparameter Pluripotency Analysis Using Magnetic Bead Assays

    Wednesday, August 28, 2013
    Application Note
    Nikolai Stankiewicz Thomas Siegl Frederick Wiese
    For the application of induced pluripotent stem cells (iPSCs) and human embryonic stem cells (hESCs) to regenerative medicine and drug discovery research, it is crucial to characterize the identity ... read more

  • Impact of New Pipetting Techniques on Safety in the Laboratory

    Monday, September 02, 2013
    Application Note
    Bobby Chavli Annette Summers Mary Napier
    According to the Occupational Safety and Health Administration (OSHA), on average, it takes between 18 and 36 months to develop and implement a new regulation, trailing the pace of science. As a ... read more

  • Single-Cell RNA Detection to Identify Cell Subpopulations in Heterogeneous Mixtures

    Tuesday, March 25, 2014
    Application Note
    Don Weldon Victor Koong Alex Ko Yuko Williams Laura White
    Traditional RNA detection methods, which analyze RNA levels in lysed, permeabilized, or fixed cell samples, make it challenging to understand the role of one gene in the context of a tissue, organ, or... read more

  • DNA in Forensics

    Wednesday, August 28, 2013
    Technical Article
    Robert L. Stevenson, Ph.D.
    The proliferation of forensic DNA testing and databases by state and local jurisdictions was recently reported by Joseph Goldstein in The New York Times. For years, the FBI’s combined indexing system ... read more

  • Improved Long-Term Culture Using A Microfluidic Platform

    Monday, October 07, 2013
    Application Note
    Alex Mok Paul Hung Cindy Chen Sónia Gil Kathleen Ongena
    Precise and biologically relevant in vitro studies require the ability to analyze cell responses to perturbations over time. In order to achieve successful long-term cell culture and imaging, however,... read more

  • Use of Integrated Data Analysis to Gain an Advantage in Biomarker Discovery and Personalized ...

    Saturday, October 01, 2011
    Technical Article
    Jens Hoefkens
    The genomic revolution of the last two decades has provided pharmaceutical companies with an almost overwhelming number of potential drug targets. read more

  • Sample Processing at Pittcon® 2014

    Tuesday, May 06, 2014
    Application Note
    Robert L. Stevenson, Ph.D.
    The tenfold increase in speed for the analytics (GC, HPLC, and SFC [supercritical fluid chromatography], all with MS) over the last decade has shifted the choke point in many workflows from analytics ... read more

  • Micro RNA Profiling with a Multiplexed Assay

    Monday, March 03, 2014
    Application Note
    Mark Santos
    From the discovery of new miRNAs to clarifying their biological roles and utility as biomarkers, micro RNA (miRNA)-based research is undergoing a period of change. Rigorous methods are therefore ... read more

  • Product Intelligence: Solid Phase Extraction Systems

    Monday, February 25, 2013
    Technical Article
    Solid phase extraction, or SPE, is used to isolate pollutants in water and pesticides in food, isolate drugs in blood and tissue samples, and separate proteins from a mixture, among other applications... read more

  • How Solid-Phase Extraction Affects Challenges in Bioanalysis

    Wednesday, February 05, 2014
    Application Note
    Mike Oliver Tim Liddicoat Ken Meadows
    Modern bioanalytical laboratories are tasked with providing high-quality analytical results from complex biological samples in a high-throughput environment. These needs have to be balanced with the ... read more

  • Optimizing HPLC Sample Preparation

    Monday, February 06, 2012
    Technical Article
    Caitlin Smith
    Sample preparation is an important component for analysis in high-performance liquid chromatography (HPLC)/ultra high-performance liquid chromatography (UHPLC). This article outlines commonly used ... read more

  • Highlights and Challenges in Laboratory Automation: Review of the 4th Annual Automation User Group ...

    Thursday, February 09, 2012
    Technical Article
    Robert L. Stevenson, Ph.D.
    For four years now, Agilent Technologies (Sunnyvale, CA) has hosted an annual users’ conference on laboratory automation. read more

  • Product Intelligence: Thermal Cyclers: Behind the Technology

    Friday, June 28, 2013
    Technical Article
    Caitlin Smith
    Also referred to as thermocyclers, DNA amplifiers, or PCR machines, thermal cyclers are a vital part of the molecular biology laboratory. Many choices of thermal cycler are available, and this article... read more

  • Translational Medicine and the ’Omics Electronic Lab Notebooks

    Tuesday, August 06, 2013
    Technical Article
    Chris Molloy
    Translational medicine research (TransMed) is a new environment in which groups of stakeholders come together to review increasingly complex data with the goals of improving outcomes, getting more ... read more

  • High-Resolution Separation of Nucleic Acid Fragments

    Wednesday, August 07, 2013
    Application Note
    Carola Schade
    The invention of the polymerase chain reaction (PCR) in the early 1980s revolutionized molecular biology and molecular medicine. Since then, PCR has developed into a widely used technique for ... read more
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