Product Intelligence: UHPLC: Pushing the Limits of HPLC

Ultra high-performance liquid chromatography, or UHPLC, is faster and provides higher resolution compared to conventional HPLC. This article discusses UHPLC operation, from sample through detection, and outlines important specifications to consider prior to purchase. Key points of consideration include type of UHPLC detector, i.e., UV-VIS, photodiode array, fluorescence, and single and tandem quadrupole MS; column pressure and diameter; sorbent; and whether to use core-shell particles.

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