ApoTome.2 Optical Sectioning using Structured Illumination

ApoTome.2 provides an economical solution for acquiring fluorescent images of thicker samples.  It works with structured illumination and allows optical sections of specimens to be obtained. A grid pattern is projected into the focal plane, three images with different grid positions are acquired and merged online, resulting in an optical section of the specimen focal plane with no residual stray light. Stacks of the 2 dimensional optical sections can be rendered in 3 dimensions with software and animations can be exported as video clips.