Optimised System for Preparation of Drosophila Melanogaster (fly) Food

8 October 2013: Researchers at the Centro Biología Molecular Severo Ochoa (CBMSO) in Madrid, Spain have demonstrated how the MEDIACLAVE Media Preparator and the DOSE IT Peri­staltic Pump from INTEGRA are able to signifi­cantly en­hance the efficiency and re­produci­bility of fly food preparation.

Drosophila melanogaster, the common fruit fly, is a commonly used model research organism in genetics, physiology and evolutionary develop­ment biology. Successful experiments using Dro­sophila typically depend on optimal husbandry and on providing good fly food quality. In some Droso­phila labs, hun­dreds of vials are filled each day with fly food.

Mer­cedes Dávila, Head of Media Preparation Services at CBMOS com­mented, “Using a basic media cooking pot approach we could not accurately control the tem­perature of our ‘fly food’ during preparation and had to stir the mixture manually using a ladle. Because of the high viscosity of the fly food our technicians found filling all the vials a time-con­suming and tedious process and often the food quality vial to vial was not consistent”. She added “Our subsequent purchase of a stirrer kettle helped to improve the fly food mixture quality. However, prepar­ing the food was still much too slow because it took several hours to cool the mixture to dis­pensing tempera­ture”.

CBMSO’s investment in an INTEGRA MEDIACLAVE 30 Me­dia Prepa­rator and a DOSE IT peristaltic pump finally was the solution to this problem. Reflecting on the advantages that CBMSO had gained from the INTEGRA system - Mer­cedes Dávila said, “Our MEDIACLAVE 30 gives us the possi­bility to pre­pare 30 litres of fly food in only 95 minutes. The integrated cooling system makes the process much faster than pre­vious solutions”. She contin­ued, “We can now keep the fly food mixture at the de­sired dispensing tem­perature. This allows us to dis­pense the food di­rectly from the cuvette into the vials, without risking contamination or premature solidifi­cation inside the dispensing tubing.” Concluding Mer­cedes Dávila said “The electronic or paper process docu­mentation from the INTEGRA system has enabled us to have stan­dardized methods and fully traceable quality”.

To watch the MEDIACLAVE and DOSE IT being used for preparing fly food please visit http://www.youtube.com/watch?v=TE57dd2QlOs&feature=youtube

For further information on the MEDIACLAVE 30 media preparation system and DOSE-IT peristaltic pump please visit www.integra-biosciences.com/sites/pdf/sterilisation/Fly_food_preparation_V01.pdf or contact INTEGRA on telephone +41-81-286-9530 / email info@integra-biosciences.com

INTEGRA Biosciences is a leading provider of high-quality laboratory tools for liquid handling and media preparation. The company is committed to creating innovative solutions which fulfil the needs of its customers in research, diagnostics and quality control within the life science markets and medical industry. Today, INTEGRA innovative laboratory products are widely used all around the world. More than ninety distribution-partners form a worldwide sales network providing responsive and competent services to customers. These distribution partners are supported by a highly motivated and experienced team of specialists at the company headquarters in Zizers, Switzerland and Hudson, NH, USA. INTEGRA is an ISO 9001 certified company.

The Centro Biología Molecular “Severo Ochoa“ (CBMSO) is a joint venture between the Spanish Na­tional Research Council (CSIC) and the Autono­mous University of Madrid (UAM). The main activities of the institute are scientific research in the fields of biology, biomedicine and teaching.


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