Oakland, California. 29 November 2012 – Rainin Instrument, a METTLER TOLEDO subsidiary, has published a new white paper describing the optimization of protein/antibody purification. The paper, entitled “Optimizing Protein Purification Conditions,” highlights the use of Rainin PureSpeedTM Protein Tips. Using Rainin’s E4 XLS electronic pipette, PureSpeed tips allow for bidirectional sample flow over the purification resin bed, maximizing target protein capture by allowing the binding reaction to reach equilibrium. Potential concentration steps, which can actually degrade protein quality, are eliminated, saving time and costly rework.
To demonstrate the process, a 6-His-tagged protein and s 20 µL Ni-IMAC PureSpeed tips were used with a Rainin E4 XLS pipette. Back-and-forth capture flow was used to ensure complete resin exposure during all steps. Concentrations of imidazole in the wash buffer and final elution were varied to help illustrate purification optimization, targeting both quantity and purity of captured protein. The experiments illustrate that PureSpeed wash and elution chemistries, which are carried out at reduced volumes, generalize to larger scale preparative systems, making PureSpeed a cost-effective optimization tool.
The paper also reviews aspects such as protein size that can affect the capture equilibrium as well as ways to help enhance protein capture by adjusting pH and flow rate or increasing cycle numbers. Rainin’s ColorTrakTM Guide also proved useful at helping non-expert operators load and purify a specific protein or antibody.
For more on how Rainin and proprietary PureSpeed technology can help optimize protein purification processes for time and cost savings, download the complete white paper at http://us.mt.com/us/en/home/supportive_content/White_Papers/product-organizations/pipe/optimizing_protein_purification_conditions.html.
About METTLER TOLEDO
Rainin Instrument, LLC, is a wholly owned subsidiary of METTLER TOLEDO, a leading supplier of precision instruments and services worldwide. Rainin pipettes have long been the largest selling brand in North America and consistently rank among the top premium pipette brands in Europe, Asia and the Middle East. METTLER TOLEDO is the world’s largest manufacturer and marketer of weighing instruments for use in laboratory, industrial and food retailing applications. It holds top three market positions in several related analytical instruments and is a leading provider of automated chemistry systems used in drug and chemical compound discovery and development. The company is also the world’s largest manufacturer and marketer of metal detection and other end-of-line inspection systems used in production and packaging. Additional information about Rainin and METTLER TOLEDO can be found at www.mt.com.
Global Marketing Communications Manager